Hydrolases in Vacuoles from Castor Bean Endosperm
نویسندگان
چکیده
منابع مشابه
Hydrolases in vacuoles from castor bean endosperm.
Vacuoles were prepared from endosperm tissue of 4-day-old castor bean seedlings (Ricinus communis var. Hale) and purified on a stepped sucrose gradient. It was shown by assays of marker enzymes that there was only trace contamination of the final preparation by other organelles (mitochondria, glyoxysomes, nuclei, spherosomes, and plastids) and by cytoplasmic components. Hydrolytic enzymes (acid...
متن کاملHydrolases in Vacuoles from Castor Bean
Vacuoles were prepared from endosperm tissue of 4-day-old castor bean seedlings (Ricinus communis var. Hale) and purified on a stepped sucrose gradient. It was shown by assays of marker enzymes that there was only trace contamination of the final preparation by other organeiles (mitochondria, glyoxysomes, nuclei, spherosomes, and plastids) and by cytoplasmic components. Hydrolytic enzymes (acid...
متن کاملpH in Vacuoles Isolated from Castor Bean Endosperm.
Vacuoles were prepared from germinating castor bean endosperm (Ricinus communis var Hale) and purified by filtration through a cotton layer under physiological osmolarity. The purity of vacuoles prepared by this method was comparable with that prepared by a sucrose step gradient centrifugation reported in a previous paper (Nishimura, Beevers 1978 Plant Physiol 62: 44-48). It was shown by assays...
متن کاملQuinolinic Acid Phosphoribosyltransferase from Castor Bean Endosperm
Quinolinic acid phosphoribosyltransf erase was purified WI-fold from the endosperm of etiolated seedlings of Ricinus communis L. Sodium dodecyl sulfate disc gel electrophoresis indicated that the enzyme was homogeneous but on analytical disc gel electrophoresis at pH 7.0 and 8.3 the isolated native protein exhibited three closely migrating bands. The three proteins were of the same molecular we...
متن کاملPhosphatidylethanolamine synthesis in castor bean endosperm.
Phosphatidylethanolamine synthesis by CDP-ethanolamine:1,2-diacylglycerol ethanolaminephosphotransferase (EC 2.7.8.1) from the endoplasmic reticulum of castor bean (Ricinus communis L. var. Hale) endosperm was characterized. The Michaelis-Menten constant of the enzyme for CDP-ethanolamine was approximately 8.0 micromolar. The pH optimum was 6.5 and a divalent cation was an absolute requirement ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Physiology
سال: 1978
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.62.1.44